Agrisera/TIP2;1 | tonoplast intrinistic protein 2-1/AS09 509/-免疫在线蚂蚁淘旗下平台-

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Agrisera/TIP2;1 | tonoplast intrinistic protein 2-1/AS09 509/

来自 : 发布时间：2025-04-04


product information
Background		TIP2-1 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.
Immunogen		KLH-cionjugated peptide derived from Oryza sativa TIP2-1 Q7XA61
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format
Quantity		100 µl
Reconstitution
Storage		store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications		ELISA (ELISA), western blot (WB)
Related products		collection of antibodies to tonoplast proteins AS09 492 \| anti-TIP1;1, TIP1;2 \| tonoplast intrinistic protein 1-1, 1-2 (gamma) AS09 493 \| anti-TIP1;1, TIP1;2 \| tonoplast intrinistic protein 1-1, 1-2 (gamma) AS09 494 \| anti-TIP2;1 (delta) \| Delta-VM23 AS09 510 \| anti-TIP2;1 \| tonoplast intrinistic protein 2-1 AS09 510 \| anti-TIP2;2 \| tonoplast intrinistic protein 2-2 AS09 511 \| anti-TIP2;2 \| tonoplast intrinistic protein 2-2 (C-terminal) Plant protein extraction buffer Secondary antibodies
Additional information		0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. Vacuolar membrane protocol is available here.

application information
Recommended dilution		1: 8000 (ELISA), 1: 2000 with standard ECL (WB), immunolocalization (IL)
Expected \| apparent MW		24.8 \| 20-24 kDa
Confirmed reactivity		Oryza sativa, Populus trichocarpa x deltoides, clone H11-11
Predicted reactivity		Gladiolus hybrid, Hordeum vulgare, Triticum aestivum, Zea mays
Not reactive in		Arabidopsis thaliana
Additional information		Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC. Manufactured by Operon Biotechnologies.
Selected references		Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

application example

1.5 µg of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP2;1 antibodies (AS09 509, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.

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本文链接： http://nist.immuno-online.com/view-1546330515.html

发布于： 2025-04-04 阅读（）

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