PIP2;2 is a plasma membrane aquaporin. Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2 proteins: AtPIP2-1, At3g53420 , AtPIP2-2 P43287, At2g37170 , AtPIP2-3 P30302, At2g37180

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

collection of antibodies to plasma membrane proteins

AS09 488 | anti-PIP2;1 | aquaporin PIP2;1

AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins

AS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2b

Plant protein extraction buffer

Secondary antibodies

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for plasma membrane isolation can be found here.